![]() This is the only watch series that has a committed enough following to foster a truly vibrant app scene. That risks glossing over what makes it so special. The Apple Watch Series 7 is generally considered the default smartwatch, the obvious choice if you have the money to spend. Whereas Fitbits are renowned for having awesome battery capacity, up to ten days in the case of the Fitbit Inspire 2.īecause picking a fitness tracker can be a minefield, the Live Science team has picked the best on the market, so you can make an informed decision before you buy. ![]() Higher end trackers like the Fitbit Sense and the Amazfit GTR 3 come with GPS as standard, and the more expensive ones tend to have bigger screens with better graphics and advanced sensors, like electrocardiograms (which check for potential heart problems).īattery life is also crucial – if you use your Apple Watch Series 7 to track sleep overnight, then you’ll find it needs charging again by morning, for example. When you’re looking for the best fitness wearable, there are a few things to consider. What is the best place to wear a tracker? You don’t just have to wear yours on your wrist as now they come in the form of rings, jewelry and even inner soles for sneakers. You can buy a basic tracker for under $10, or spend from hundreds to thousands of dollars on the latest tech. ![]() ![]() As well as basic metrics and tracking steps, the latest fitness trackers and smart wearables are packed with features to help you monitor and improve your health, fitness and wellbeing – and there are models to suit every need and budget. ![]()
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![]() The franchise includes various doll lines, media tie-ins, and licensed merchandise. Equestria Girls features anthropomorphized versions of My Little Pony characters from that period as with My Little Pony, which features a colorful body and mane, Equestria Girls characters sport non-human skin and hair colors, while incorporating corresponding pony counterpart's cutie marks (the flank symbol) in their clothing. My Little Pony: Equestria Girls, simply known as Equestria Girls or EQG, is a product line of fashion dolls and a media franchise launched in 2013 by the American toy company Hasbro, as a spin-off of the 2010 relaunch of the My Little Pony line of pony toys and its Friendship Is Magic television series.
![]() Members of the Reeves Grove Historical Committee placed white ribbons on the graves of the church’s founders.Īlthough most scientists are skeptics, Gregg says dowsing has been proven many times. Modern dowsing begins to appear in records around the 15th century in Europe. In a self-published booklet titled, Dowsing for Fun and Profit, Gregg writes, “In Peru, a rock carving more than 9,000 years old depicts a man holding a forked dowsing stick,” he writes. It was amazing! There were some people there who couldn’t get the hang of it, or it didn’t work for them, but for Joseph and me and one other lady, it did.” Somehow it seemed to work for myself and Joseph Williams, also from Springville and part of the Springville Preservation Society. “He gave a long, interesting class that morning, we had lunch, then went to an old cemetery,” Waid says. She is a member of area historical and genealogical societies and thought grave dowsing might add depth (no pun intended) to that type of research. ![]() Waid took a grave-dowsing class from Gregg about three years ago at his wife’s urging. “Most of these slaves came from South Carolina, and they were Caribbean slaves, so some of their beliefs determined how they were buried.” “They wouldn’t have had the resources for caskets, so they probably wrapped the bodies in blankets,” he said. Waid noticed that all the graves in the slave section were small and wondered whether they might be buried in a fetal position. Wayne can tell that, but I don’t have that experience yet.” Lots of times (during the 1800s) women died in childbirth and they buried mom with her baby on her chest. “If they cross high, it’s a female if low, it’s a male. “I use two wires I got from Wayne,” Waid says. Gregg narrated while Waid demonstrated dowsing techniques. The Backwood Boys – Gallant musicians Adron and Mark Willingham on guitars, Marlin Galloway on mandolin – continued to play as the audience filed outside behind Gregg and Waid for the actual grave dowsing. “They also enjoyed Macki Branham’s brief history of the church and Glenda Tucker showing off a fashionable dress from the McCorkle era.” The dress was once worn by Eliza Elizabeth (Moore) Keith, (December 16, 1827-January 8, 1891), the great-great-grandmother of Glenda’s husband, Harry Tucker. ![]() People were so interested in Gregg’s presentation on grave dowsing that their questions and his answers pushed his allotted 30-45 minutes on the program to an hour. The church historical committee is restoring the sanctuary and its attached fellowship hall to be used as an event venue.īut restoration wasn’t uppermost in the minds of those in attendance on dowsing day. The dowsing event was another effort to raise awareness and money for the restoration of the original church building. I think there’s two people in this grave, but I’m not sure, because I’m not as experienced as Wayne Gregg.’ So, when they had the special day and demonstrated dowsing for the people, Wayne started walking in the spot where I had been walking and said, ‘There are two people buried here!’” I was getting readings of male and female bodies. (Moyer says they put ribbons on the graves of the church founders.) One kept giving all kinds of problems. ![]() “We laid out the McCorkle Cemetery and marked a bunch of graves with flags. “I went up there one afternoon with some members a week before they had the official event,” says Frank Waid, a Springville grave dowser who studied under Gregg. Gregg, who is from DeKalb County, wasn’t the first dowser to notice the anomaly of an extra person in a grave, however. The committee wants to mark those graves and give the folks buried there the recognition they are due, even if their names are unknown. The group soon moved on to the slave section of the cemetery, where the graves have no stones. “We think it might have been Elizabeth’s husband,” says Linda Moyer, chairperson of the Reeves Grove Historical Committee. His rods indicated the presence of a second body that wasn’t represented on the grave marker. ![]() The markers weren’t where they were supposed to be. According to the marker, that grave belonged to Elizabeth McCorkle, wife of the original owner of the McCorkle Plantation upon which Reeves Grove now stands. It didn’t take long for Wayne Gregg’s rods to cross, indicating a grave. Backwood Boys Mark Willingham, Marlin Galloway and Adron Willingham play at grave dowsing event. ![]() ![]() ![]() Shorter incubation time with detection substrate. Reusing blocking buffers is not advisable. Using pure protein such as BSA or casein as a blocker can help. Different blocking agents may help(gelatin, BSA, albumin, casein, nonfat dry milk) Increase of temperature at which blocking is performed. Increase of duration of the blocking step, for example 8 hours at 4C instead of an hour at room temperature. Concentration of blocker can be increased(3-5% BSA, casein or nonfat dry milk). Following the protocol and careful handling ensures that the experiment runs smoothly, but several trials may be needed to resolve this problem. If high background is seen across the blot, several things may be the reason. The easiest way to counter it is to prewet the membrane in methanol before equilibration in aqueous transfer buffer and once it is wet, do not let it dry out. White regions on PVDF membrane show areas where the membrane was either incorrectly wetted or had the time to dry out. After the sheet is completely hydrated, it is left in transfer buffer until it is ready to use. If sheet is not immediately immersed in transfer buffer in order to wet it, distilled water should be heated up just below 100C and the sheet should be left to hydrate inside the water. White regions on the nitrocellulose membrane show dry regions where protein is unable to bind. Chilling the buffers, using cooling coil or "blue ice" insert in the cell can help with temperature regulation. ![]() ![]() You can do the transfer in tank placed on an ice bath or temperature-regulated room (4C) Ensure that the buffer is cool by reducing the current and increasing transfer time to compensate Tank blotting with high-intensity transfers can lead to heating the buffer and formation of bubbles: Consider that collodial gold and coomassie are not compatible with downstream steps. If you want to use the blot in downstreams steps, confirm that the stain can be removed or its compatibility with antibody antibody detection. Running a diagnostic test like checking transfer quality via imaging proteins on the gel and the blot is advisable. You can visualize total protein on blots or gels using stain-free gels. In order to be sure that the transfer is successful, staining the membrane with Ponceau S after blotting is advisable. Checking for residual protein or protein unable to transfer, remaining on the gel, using a total protein stain is advisable after transfer is complete. If you use a tank blotter, make sure that the sponges(foam pads) can create a proper assembly and if they can no longer create a good seal, the sponges should be replaced with new ones. Using a rapid transfer pack greatly helps preventing improper sandwich assembly. Carefully remove all air bubbles present between the membrane and the gel prior to transferring the protein, either a glass rod or a roller can be used. Causes:įaulty assembly of transfer sandwich, air bubbles are present. Both can be easily prevented with improving your technique and additional care. White areas on WB background can be caused by air bubbles or dry areas of membrane restricting proteins from binding.
![]() In 2020, the Grateful Dead released an animated music video for “Ripple” in honor of American Beauty‘s 50th anniversary. With Jerry Garcia composing the thoughtful acoustic arrangement to accompany this, and of course providing his angelic voice on lead vocals, we are left with a masterpiece that invites one to ponder and reflect upon their own place in the universe. That path is for your steps alone Lyrics from “Ripple” by the Grateful Dead In this way Hunter places “Ripple” in context with all of these things that weigh and interpret the meaning of life, and gives his own interpretation, but moreso invites listeners to ponder it for themselves: ![]() The Grateful Dead playing acoustic in 1970. Noted references include the ancient Indian mystic Kabir, the Hebrew Psalm 23, Walt Whitman and Yeat’s poetry, and more. It suggests rather directly that there is some greater being out there that created a mystical fountain, which one might assume is the Fountain of Youth.Īs noted in the old school annotated Grateful Dead lyrics site at the University of Santa Cruz, Hunter’s lyrics also contain what could be interpreted as references to several other works of mythology and literature. Especially with the line, “Let it be known there is a fountain / That was not made by the hands of men,” which Hunter told Rolling Stone in 2015 was his favorite lyric that he had ever written. “Ripple”, however, certainly does dip its toes in the waters of religion, so to speak. This same line of thinking is why he shot down the idea of the band members holding guns on the back cover of Workingman’s Dead. ![]() I’m sure he was also considering the copious amounts of LSD that both the band and its fans were taking around this time. ![]() He noticed from early on that the fans were being influenced by the band and their music, and he didn’t want anybody to get the wrong idea. Robert Hunter was known to purposely avoid too many religious or anything that could be interpreted as radical undertones with his songwriting. The Dead embodied the spirit of this campfire anthem with the family that they created, based on a foundation of love and peace and respect. It’s about remembering that we’re all in this together, but also that your life is your own to lead. “Ripple” is about making peace with life, and finding a path that suits you by learning from the other lost people around you, and of course embracing the power of joy through music. This was the songwriting duo that produced many of the Grateful Dead’s best songs, including most of the material on their two most beloved albums, American Beauty and Workingman’s Dead, and other favorites like “Sugaree”. The lyrics to “Ripple” were written by Robert Hunter, with music composed by Jerry Garcia. It is rumored that the reason the Dead played the song that night in ’88 was because the Make-A-Wish Foundation approached them with the wish of a deadhead who was dying of cancer, though this has never been confirmed. “Ripple” was shelved again in May of ’81 and did not return until 1988, making one final appearance as an electric encore that nobody could have expected. Then it was dropped from the rotation for nearly a decade until it reappeared when the band brought back acoustic sets in 1980-81.Ĭheck out a video of the Dead performing “Ripple” at Radio City Music Hall, Halloween 1980 below. The Dead only performed “Ripple” live 40 times, beginning in August 1970 and continuing during acoustic sets throughout that year and into some electric sets in ’71. The American Beauty version simply shines, with Jerry, Bob Weir, and Phil Lesh all nailing the harmonies, which is much key to this song and proved much more difficult for them to accomplish in the live setting. It’s one of only a handful of Dead songs that you could make a reasonable argument about the studio version being better than any of the live versions ( “Touch of Grey” and “Box of Rain” also come to mind). ![]() They contain many of the Dead’s most memorable songs, including “Ripple”. These albums marked a shift in their sound from the psychedelic madness they were exploring in the late 60s into a more roots-oriented rock sound that they stuck with for nearly a decade, and never truly strayed too far away from. The tune was released on the Grateful Dead’s 1970 album, American Beauty, which was the second of two noteworthy albums that they released that year, the first being Workingman’s Dead. And you’d be hard-pressed to find a deadhead who wouldn’t place “Ripple” in the running for their favorite Dead songs. With it’s deep, pondering lyrics penned by Robert Hunter and the peaceful acoustic arrangement by Jerry Garcia, “Ripple” is a song that’s meaning boils down to the pure essence of the Grateful Dead. Out of all the wonderful songs composed by the Grateful Dead, “Ripple” is one that seems to have a special magic. The Grateful Dead at Radio City Music Hall in October 1980. ![]() ![]() ![]()
![]() The main character of Is This Order a Rabbit, Chiya is a kind, upbeat girl with an overactive imagination she surprisingly likes creepy stories and strives to become a samurai. 4 Chiya Ujimatsu (Is This Order A Rabbit?) But out of all of them, Aria is the closest to her childhood friend Shino Amakusa, with the two sharing the same lecherous sense of humor. RELATED: Things Many Anime Exaggerate About Student CouncilsĪria is well-respected among the Student Council, with many members looking to her as a parental figure. Although she comes from wealth, she has no qualms about using her family's vast fortune to fund various activities and expenses, though she is naive because of her background as well. The secretary of the Student Council, Aria happily supports the Student Council as well as its members to the best of her ability. Combined with her admirable intelligence, Eru ensures that any mystery that comes her way will never go unsolved. Together, the three of them investigate numerous happenings in and out of school, with Eru's mystery-oriented drive being the glue that drives the trio forward. She is also extremely adventurous, always excited to solve whatever mystery comes her way, serving as a stark contrast to Hotaro's lethargic personality.Īlongside Hotaro and Satoshi, Eru is one of the three founding members of the Classic Literature Club. Like Yukiko, Eru grew up in a well-to-do environment and was brought up to be a proper lady. Although she assists in the many assassination attempts on Koro-sensei, Yukiko ultimately chooses to spare his life, as she views him as her mentor. Noted by others as the most beautiful girl in their class, Yukiko spent her entire life being raised to be a proper lady. RELATED: The Best Anime About Serial Killers As a result, she is often the victim of unwarranted flirting from her male peers. Despite this, Yukiko can be rather meek, especially when it comes to men. One of many students in Koro-sensei's class, Yukiko is one of his more mature students, retaining a refined and composed demeanor. 7 Yukiko Kanzaki (Assassination Classroom) In an effort to be with Kyosuke, Manami takes it upon herself to sabotage all of Kyosuke's other relationships before ultimately choosing to support him and whoever he chooses as his girlfriend. Manami's most defining aspect is her obsessive love for Kyosuke, whom she has known since they were both children. She may seem shy and introverted at first, but when pushed to the limit, Manami can become very blunt and mean, much to the shock of those in the vicinity. Manami may be plain when it comes to her appearance, but her personality is extremely dynamic. Her personality would eventually clash with her rotorella during the school play, so much so that the script was rewritten in order to fit her standoffish nature. Nowadays, Saki is still quite reserved, but she likes to spook people with her gothic appearance and wave ability. RELATED: Every Main Character's Age, Zodiac, and Height in Fruits Basket Naturally, her devotion to Tohru is second to none, to the point that she admits that Tohru is her only weakness. 9 Saki Hanajima (Fruits Basket)Īfter relentless bullying throughout her childhood due to being perceived as a witch, Saki finally received acceptance for who she was upon meeting Tohru Honda. Afterward, she becomes more sympathetic towards the people of Wano and is fully subservient to Tama, forming a mother-and-daughter relationship with her. ![]() In the One Piece anime, Speed briefly fought Zoro and Kiku in Bakura Town before being tamed by Tama's Kibi Kibi no Mi powers. ![]() As a horse SMILE user, she possesses numerous horse-like characteristics like her centaur appearance and her comical horse smile. Speed first appeared as one of the Shinuchi working under Kaido she assisted in hoarding the food and clean drinking water for herself and the other animal-themed Beast Pirates. With an overall prolific presence in entertainment, her work has yielded some of the best performances in anime, with ten roles, in particular, standing out among the rest. Sato's voice work has also lent itself to a successful musical career she has performed songs related to the characters she has voiced. P> Satomi Sato is one of anime's most recognized anime voice actors, appearing in top-rated shows such as Fruits Basket and Fairy Tail, she is able to voice a diverse range of character types, from those that are cute and bubbly to others that are doom and gloom. ![]() ![]() ![]() The DCO has preprogrammed control of the individual harmonics within the waveform, and some of (but not all of) the available waveforms are "moving", meaning that the amplitudes of the various harmonics are designed to change as the DCA envelope progresses. The SD sound source is a 4-bit digitally-controlled oscillator (DCO) that uses preprogrammed additive synthesis to offer waveforms including triangle, sawtooth, squares of different widths, and some unusual pulses, plus various combinations thereof. SD (Spectrum Dynamic) Synthesis was modeled on traditional DCO-VCF-DCA analog synthesis but used waveforms that included predefined variations over time. The subsequent SD syntheizers were marketed as advanced home keyboards (i.e., including speakers and programmable accompaniment) launched under the HT prefix (with the notable exception of the non-editable, preset-only Casiotone version, the MT-600). The 1987 Casio HZ-600 was the initial model and was considered an entry-level offshoot of the "Z" series of synthesizers that included the CZ and VZ lines. Yamaha's then-active lawsuit against Casio's PD synthesis method (which claimed PD synthesis infringed on Yamaha's patented Frequency-Modulation synthesis), may also have contributed to Casio's development of SD synthesis to diversify their offering, even while they expanded upon PD synthesis in the VZ line of synths. In turning to SD synthesis Casio meant to create a synthesis engine that was more comprehensible and accessible than Phase Distortion synthesis. When Casio decided to retire the CZ line, they decided to go in two directions: more complex (the VZ "Interactive" Phase Distortion line), and more traditional (SD synthesis, starting with the HZ-600). SD Synthesis followed on the heels of the more advanced Phase-Distortion (PD) Synthesis employed in the successful line of Casio CZ synthesizers. There still exists a small but devoted fanbase who insist that SD synthesis, particularly as expanded in the high-end model HT-6000, was overlooked and highly underrated. Due to some programming limitations plus Casio's poor marketing, the SD synths never gained wide popularity and are now fairly rare in the second-hand marketplace (which adds to their charm, according to some). SD synthesis is used in six Casio synthesizers and home keyboards released in 1987 and produced until 1991, when Casio exited the synthesizer market completely and focused solely on pure consumer keyboards. SD synthesis was very similar to traditional DCO-analog synthesis, with the main difference being that some of the SD digital waveforms' harmonic spectrums changed temporally, or dynamically in relation to the amplitude envelope. (March 2008)Ĭasio's SD ("Spectrum Dynamic") Synthesizers were a late-1980's line of hybrid digital-analog synthesizers featuring a resonant analog filter. Unsourced material may be challenged and removed. Please help improve this article by adding citations to reliable sources. ![]() This article does not cite any references or sources. ![]() ![]() In case a driver's license is required for the role, also mention it. Also mention your place of residence and address, as an employer might prefer an employee living nearby. Therefore, you always start by mentioning your full name and email address and (mobile) phone number. Of course, your new employers should be able to contact you for a job interview. 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